primary mouse monoclonal anti human fetuin a ahsg antibody (R&D Systems)
Structured Review

Primary Mouse Monoclonal Anti Human Fetuin A Ahsg Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary mouse monoclonal anti human fetuin a ahsg antibody/product/R&D Systems
Average 93 stars, based on 7 article reviews
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1) Product Images from "Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468"
Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468
Journal: Journal of pharmacy and pharmacology research
doi: 10.26502/fjppr.0103
Figure Legend Snippet: Overexpression of fetuin-A (Fet-A) in MDA-MB-468 TNBC cell line. MDA-MB-468 cells were transfected with either empty vector (EV) or flag-tagged fetuin-A expression vector (FA). Fetuin-A expression level was determined by QT-RTPCR (panel A), Western blot (panel B) and IHC (panel C) as described in .
Techniques Used: Over Expression, Transfection, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot
Figure Legend Snippet: Enhanced invasion capacity in fetuin-A overexpressing MDA—MB-468 cells. The MDA-MB-468-FA invaded through a bed of Matrigel more readily compared to MDA-MB-468-EV when complete medium (CM) was in the lower compartment of the trans-well (*P<0.05); N=6). Both sub-clones did not invade when SFM was in the lower compartments (controls) (**P<0.001; N=6)
Techniques Used: Clone Assay
Figure Legend Snippet: Toll like receptor 4 (TLR4) expression in MDA-MB-468 sub-clones. Surface TLR4 expression levels were determined by flow cytometry in MDA-MB-468-EV (upper panels) and MDA-MB-468-FA (lower panels) in the absence and presence of added fetuin-A (FA)
Techniques Used: Expressing, Clone Assay, Flow Cytometry
Figure Legend Snippet: Fetuin-A mediates 2-D and 3-D growth of MDA-MB-468 via TLR4 signaling. The cells were seeded (2 × 10 4 cells/well) in attachment (2-D) ( panel A ) or ultra-low attachment (3-D) ( panel B ) 96-well plates in SFM; SFM + CLI-095 (CLI); Fetuin-A (FA); FA + CLI; complete medium (CM); and in CM + CLI. After 6 days of growth, Alamar blue was added to each well, incubated for 2 h and fluorescence measured as described. *P< 0.05; **P< 0.001; ***P<0.0001; N= 4
Techniques Used: Incubation, Fluorescence
Figure Legend Snippet: In A , control chambers had only cells in SFM, while experimental chambers had CLI-095 (28 µM) in SFM (upper chambers) of trans-well assay. The bottom chambers had complete medium (CM). In B , controls had CM only in the bottom chambers while experimental chambers had CM depleted of fetuin-A (CM + HA). Number of invading cells was determined as described in .
Techniques Used: Control